Exosomes and Microbiota: Recent Advances and Perspectives
Dr. Duncan Ross and Dr. Rainer Mutschler from Biomedical Center Speyer, Germany, will talk about Exosomes and Microbiota: Recent Advances and Perspectives.
They both confirm that exosomes, or extracellular vesicles, are nanometer lipid vesicles known to be secreted by most eukaryotic and prokaryotic cell types. Additionally, it is well known that germ free animal models lacking commensal bacteria are prone to food allergies among other types of dysfunction. The production of extracellular vesicles by gram negative and gram positive bacteria is recently being understood and are classified as outer membrane vesicles when released by Gram-negative bacteria or as membrane vesicles when released by Gram-positive bacteria. In some models, bacterial EVs alone can induce regulatory T cell formation potentially tolerizing the host to these foreign antigens. The isolation and more importantly, characterization, of these small vesicles is a rapidly evolving field. Methods such as dSTORM, High performance liquid chromatography, RNA characterization and the use of known control EVs are all critical to the creation of reproducible exosome products. Bacterial EVs can interact with host cells through three mechanisms: (1) direct activation of host receptors; (2) delivery of bacterial EV content; and (3) full incorporation of EVs into the host cell cytoplasm. TLR activation and downstream signaling will be discussed in the context of host immune responses, as well as methods of GMP manufacturing of same.
Other Speaker in the session:
Dr. Rainer Mutschler, Biomedical Center Speyer, Germany
Targeting Microbiota 2021 Congress
October 20-22, 2021 - Interactive Online Congress
www.microbiota-site.com
