Culturing the Complexity of Intestinal Microbiota In-Vitro in Bac3Gel®

Dr. Daniela Pacheco, CTO and Founder of Bac3Gel, Portugal, will share her latest research during Targeting Microbiota 2024 this October.

"Culturing the Complexity of Intestinal Microbiota In-Vitro in Bac3Gel"

Presentation Highlights

• Bac3Gel demonstrated to be successful in sustaining the complexity of intestinal microbiota in vitro, making it a powerful tool for microbiota research.
• By using Bac3Gel, researchers were able to culture in-vitro the microbiota population from the isolates of 5 adult donors, achieving greater diversity and dynamic behavior compared to traditional methods like Brain Heart Infusion Medium (BHI) for up to 72h.
• The microbiota population cultured in Bac3Gel showed sustained microbial diversity over time, including previously unculturable bacteria.
• The matrix properties of Bac3Gel favored the growth of exopolysaccharide producers and showed an increase in the viscoelastic properties of the model over time. Thus, providing insight about the capacity of the substrate to mimic the remodeling behavior of the mucosal layer.
• Metabolomics analysis of the secretome of bacteria cultured in Bac3Gel confirmed the production of key metabolites such as lactate, therefore indicating desired microbial activity.
• These results suggest that Bac3Gel is a groundbreaking innovation for sustaining complex microbiota in vitro, offering a cutting-edge solution for advancing research in gut microbiota and related health studies.

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International Society of Microbiota
Targeting Microbiota 2024 Conference
October 14-15, 2024 - Malta
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