Culturing the Complexity of Intestinal Microbiota In-Vitro in I-Mu3Gel®
Ms. Natalia Suárez Vargas, Bac3Gel, Portugal, will share her latest research during Targeting Microbiota 2024 this October.
"Culturing the Complexity of Intestinal Microbiota In-Vitro in I-Mu3Gel®"
Presentation Highlights
- I-Mu3Gel® demonstrated to be successful in sustaining the complexity of intestinal microbiota in vitro, making it a powerful tool for microbiota research.
- By using I-Mu3Gel®, researchers were able to culture in-vitro the microbiota population from the isolates of 5 adult donors, achieving greater diversity and dynamic behavior compared to traditional methods like Brain Heart Infusion Medium (BHI) for up to 72h.
- The microbiota population cultured in I-Mu3Gel® showed sustained microbial diversity over time, including previously unculturable bacteria.
- The matrix properties of I-Mu3Gel® favored the growth of exopolysaccharide producers and showed an increase in the viscoelastic properties of the model over time. Thus, providing insight about the capacity of the substrate to mimic the remodeling behavior of the mucosal layer.
- Metabolomics analysis of the secretome of bacteria cultured in I-Mu3Gel® confirmed the production of key metabolites such as lactate, therefore indicating desired microbial activity.
- These results suggest that I-Mu3Gel® is a groundbreaking innovation for sustaining complex microbiota in vitro, offering a cutting-edge solution for advancing research in gut microbiota and related health studies.
International Society of Microbiota
Targeting Microbiota 2024 Conference
October 14-15, 2024 - Malta
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